Therapeutic Effect of Cang-Ai Volatile Oil on High-Altitude Rats With Cardiac Hypertrophy Through Modulation of Oxidative Stress Response

LIANG Boshen, YIN Hongke, WANG Lei, CHEN Haotian, FANG Xin, ZHAO Sisi, ZHU Qi, XIONG Lei, SUO Jinghang, CHEN Baiju

Abstract

Objective  To explore the therapeutic effect of Cang-ai volatile oil (CAVO) on rats with myocardial hypertrophy (MH) exposed to the hypobaric hypoxic environment of the Qinghai-Tibet Plateau using 7.0-tesla (7.0T) cardiac magnetic resonance imaging (CMR).

Methods A total of 50 male specific pathogen-free (SPF) Sprague-Dawley (SD) rats were randomly assigned to a low-altitude control (CON) group, hypobaric hypoxia (HH) group, myocardial hypertrophy modeling (MH) group, MH modeling plus CAVO treatment (MH+CAVO) group, and MH modeling plus benadryl hydrochloride treatment (MH+RX) group, with 10 rats in each group. Except for the CON group, the rats in all the groups were kept and fed in the standard way for 8 weeks in a high-altitude environment (at 4250 m above sea level), and then given the corresponding treatment drugs by gastric gavage. Afterwards, 7.0T high field strength CMR was used to measure left ventricular (LV) function and myocardial strain. Hematoxylin-eosin (HE) staining and Masson staining were performed to observe myocardial interstitial fibrosis. Wheat germ agglutinin (WGA) staining was performed to analyze the cross-sectional area of cardiomyocytes. Transmission electron microscopy was used to observe the ultrastructural changes of the myocardium. Serum levels of cardiac troponin T (cTnT), superoxide dismutase (SOD), malondialdehyde (MDA), and glutathione peroxidase (GSH-PX) were measured by ELISA.

Results  Compared with those of the control group, the MH group had significantly lower left ventricular global circumferential strain (LVGCS) at (－18.85±1.67)% and left ventricular global longitudinal strain (LVGLS) at (－20.39±1.48)% (P<0.05). However, the MH+CAVO group had significantly higher LVGCS at (－22.10±1.08)% and LVGLS at (－24.60±1.72)% compared with those of the MH group (both P<0.05), indicating that CAVO treatment improved LV function. The MH group had a decreased level of serum glutathione peroxidase (GSH-Px) in comparison with the CON group ([1173.49±27.10] U/mL vs. [300.83±47.25] U/mL, P<0.01), a decreased SOD level in comparison with the CON group ([302.27±3.65] U/mL vs. [105.96±4.03] U/mL, P<0.01), and an increased level of serum malondialdehyde (MDA) in comparison with the CON group ([57.91±1.13] μmol/L vs. [6.65±2.99] μmol/L, P<0.01), suggesting that the antioxidant capacity of rats in the MH group was decreased. After CAVO intervention, rats in the MH+CAVO group exhibited an increase in the serum levels of SOD at (278.51±5.97) U/mL and GSH-Px at (961.82±17.56) U/mL, as well as a decrease in MDA at (17.79±1.33) μmol/L (all P<0.05).

Conclusion CAVO can effectively improve cardiac function in rats with cardiac hypertrophy exposed to high-altitude environment by modulating oxidative stress and ameliorating cardiac hypertrophy.

 

Keywords: Cang-ai volatile oil, 7.0T cardiac magnetic resonance, High altitude, Hypobaric hypoxia, Myocardial hypertrophy


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References


LICHTBLAU M, SAXER S, FURIAN M, et al. Cardiac function and pulmonary hypertension in Central Asian highlanders at 3250 m. Eur Respir J, 2020, 56(2): 1902474. doi: 10.1183/13993003.02474-2019.

LUO H, LIAO X, TANG Q, et al. Traditional Chinese medicine for acute mountain sickness prevention: a systematic review and meta-analysis of randomized controlled trials. J Tradit Chin Med Sci, 2023, 10(1): 73-82. doi: 10.1016/j.jtcms.2022.11.008.

RICHALET J P, HERMAND E, LHUISSIER F J. Cardiovascular physiology and pathophysiology at high altitude. Nat Rev Cardiol, 2023, 21(2): 75-88. doi: 10.1038/s41569-023-00924-9.

YIN H K, LIANG B S, CHEN H T, et al. 7.0 T magnetic resonance imaging evaluation of left ventricular function improvement in rats with pulmonary arterial hypertension in the Qinghai-Tibet Plateau environment following sodium selenite administration: a preliminary study. Chin J Magn Reson Imag, 2024, 15(4): 126-132. doi: 10.12015/issn. 1674-8034.2024.04.020.

HAN Y, LI S, ZHANG Z, et al. Bawei Chenxiang Wan ameliorates right ventricular hypertrophy in rats with high altitude heart disease by SIRT3-HIF1α-PDK/PDH signaling pathway improving fatty acid and glucose metabolism. BMC Complement Med Ther, 2024, 24(1): 190. doi: 10.1186/s12906-024-04490-6.

YANG L, CAO J, MA J, et al. Differences in the microcirculation disturbance in the right and left ventricles of neonatal rats with hypoxic pulmonary hypertension. Microvasc Res, 2021, 135: 104129. doi: 10.1016/j.mvr.2020.104129.

LI X, PU Z, XU G, et al. Hypoxia-induced myocardial hypertrophy companies with apoptosis enhancement and p38-MAPK pathway activation. High Alt Med Biol, 2024, 25(3): 186-196. doi: 10.1089/ham. 2023.0036.

OLDFIELD C J, DUHAMEL T A, DHALLA N S. Mechanisms for the transition from physiological to pathological cardiac hypertrophy. Can J Physiol Pharmacol, 2020, 98(2): 74-84. doi: 10.1139/cjpp-2019-0566.

XU H B, SONG X N, YAN C Q, et al. Resveratrol attenuates cardiac function impairment inplateau hypobaric hypoxia rats. Chin J Appl Physiol, 2022, 38(6): 644-649. doi: 10.12047/j.cjap.6384.2022.117.

ZHANG J, XIONG Z, TIAN D, et al. Compressed sensing cine imaging with higher temporal resolution for analysis of left atrial strain and strain rate by cardiac magnetic resonance feature tracking. Jpn J Radiol, 2023, 41(10): 1084-1093. doi: 10.1007/s11604-023-01433-y.

CHEN B, LI J, XIE Y, et al. Cang-ai volatile oil improves depressive-like behaviors and regulates DA and 5-HT metabolism in the brains of CUMS-induced rats. J Ethnopharmacol, 2019, 244: 112088. doi: 10.1016/j. jep.2019.112088.

LI R Y, ZHU J, LI Y C, et al. Basic research on neuroprotective effect of volatile oil of cang ai after cerebral ischemia-reperfusion injury based on diffusion tensor imaging. J Sichuan Univ (Med Sci), 2020, 51(5): 636-642. doi: 10.12182/20200960103.

ZHANG M, ZHU D, WAN Y, et al. Using 7.0 T cardiac magnetic resonance to investigate the effect of estradiol on biventricular structure and function of ovariectomized rats exposed to chronic hypobaric hypoxia at high altitude. Arch Biochem Biophys, 2022, 725: 109294. doi: 10.1016/j.abb.2022.109294.

ZHANG X, YANG Z, SU S, et al. Kaempferol ameliorates pulmonary vascular remodeling in chronic hypoxia-induced pulmonary hypertension rats via regulating Akt-GSK3β-cyclin axis. Toxicol Appl Pharmacol, 2023, 466: 116478. doi: 10.1016/j.taap.2023.116478.

BRITO J, SIQUES P, ARRIBAS S M, et al. Adventitial alterations are the main features in pulmonary artery remodeling due to long-term chronic intermittent hypobaric hypoxia in rats. Biomed Res Int, 2015, 2015: 169841. doi: 10.1155/2015/169841.

SPYROPOULOS F, VITALI S H, TOUMA M, et al. Echocardiographic markers of pulmonary hemodynamics and right ventricular hypertrophy in rat models of pulmonary hypertension. Pulm Circ, 2020, 10(2): 2045894020910976. doi: 10.1177/2045894020910976.

IMRAY C, WRIGHT A, SUBUDHI A, et al. Acute mountain sickness: pathophysiology, prevention, and treatment. Prog Cardiovasc Dis, 2010, 52(6): 467-484. doi: 10.1016/j.pcad.2010.02.003.

WANG Q, MEI J, QI Z W, et al. Discussion on aromatic Traditional Chinese Medicine in the Treatment of cardiovascular diseases. Chin J Integr Tradit West Med, 2023, 43(9): 1117-1121. doi: 10.7661/j.cjim. 20230207.111.

HUANG R J, WEN X J, LI R Y, et al. Effects of Cang-Ai volatile oils on cardiac functions in normal rats based on 7.0T cardiac magnetic resonance. Chin Tradit Pat Med, 2022, 44(3): 746-752. doi: 10.3969/j.issn. 1001-1528.2022.03.011.

MNAFGUI K, HAJJI R, DERBALI F, et al. Anti-inflammatory, antithrombotic and cardiac remodeling preventive effects of eugenol in isoproterenol-induced myocardial infarction in Wistar rat. Cardiovasc Toxicol, 2016, 16(4): 336-344. doi: 10.1007/s12012-015-9343-x.

DILETTA M G, YLENIA D R, LUIGIA F, et al. The beneficial effect of carvacrol in HL-1 cardiomyocytes treated with LPS-G: anti-inflammatory pathway investigations. Antioxidants, 2022, 11(2): 386. doi: 10.3390/antiox11020386.

LI H C, LI M, ZHANG M X, et al. Ameliorative effects of Cang-Ai volatile oil on left ventricular remodeling in rats. J Sichuan Univ (Med Sci), 2023, 54(1): 128-135. doi: 10.12182/20230160208.

SHI F H, LI H, SHEN L, et al. Beneficial effect of sodium-glucose co-transporter 2 inhibitors on left ventricular function. J Clin Endocrinol Metab, 2022, 107(4): 1191-1203. doi: 10.1210/clinem/dgab834.

SHETYE A M, NAZIR S A, RAZVI N A, et al. Comparison of global myocardial strain assessed by cardiovascular magnetic resonance tagging and feature tracking to infarct size at predicting remodelling following STEMI. BMC Cardiovasc Disord, 2017, 17(1): 7. doi: 10.1186/s12872-016- 0461-6.


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