circFOXP1 Targets miR-4429 to Regulate Proliferation, Migration and Invasion of Nasopharyngeal Carcinoma 6-10B Cells
Abstract
Objective
To determine whether circular RNA forkhead box protein P1 (circFOXP1) regulates the proliferation, migration and invasion of nasopharyngeal carcinoma 6-10B cells by targeting miR-4429.
Methods
The correlations of circFOXP1 and miR-4429 with pathological characteristics of nasopharyngeal carcinoma patients were analyzed. Nasopharyngeal carcinoma 6-10B cells were transfected with si-NC, si-circFOXP1, pcDNA, pcDNA-circFOXP1, miR-NC, miR-4429 mimics, or co-transfected with si-circFOXP1 and anti-miR-NC, or si-circFOXP1 and anti-miR-4429. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect circFOXP1 and miR-4429 expression in nasopharyngeal carcinoma tissues. Cell viability, colony formation, migration and invasion abilities were assessed by Cell Counting Kit-8 (CCK-8), colony formation, wound healing and Transwell assays. Western blotting was used to detect E-cadherin and N-cadherin protein expression. A dual-luciferase reporter assay verified the targeting relationship between circFOXP1 and miR-4429.
Results
The expression level of circFOXP1 in nasopharyngeal carcinoma tissues (4.61 ± 0.31) was significantly higher than that in adjacent tissues (1.00 ± 0.05) (P < 0.001), while the expression level of miR-4429 (0.37 ± 0.03) was significantly lower than that in adjacent tissues (1.00 ± 0.08) (P < 0.001). The expression of miR-4429 was correlated with tumor volume and TNM stage (P < 0.05), while the expression of circFOXP1 was correlated with TNM stage(P < 0.05) but not with tumor volume (P > 0.05). Inhibition of circFOXP1 expression or overexpression of miR-4429 reduced cell viability, colony formation number, wound healing rate, invasive cell number and N-cadherin protein expression, while increased E-cadherin protein expression in nasopharyngeal carcinoma 6-10B cells (P < 0.05). circFOXP1 targeted and negatively regulated miR-4429 expression. Interference with miR-4429 reversed the inhibitory effects of circFOXP1 silencing on proliferation, migration and invasion of nasopharyngeal carcinoma cells (P < 0.05).
Conclusion
Inhibition of circFOXP1 expression can attenuate the proliferation, migration and invasion of nasopharyngeal carcinoma 6-10B cells by targeting miR-4429.
Keywords: Nasopharyngeal carcinoma 6-10B cells, circFOXP1, miR-4429, Proliferation, Migration,Invasion
Full Text:
PDFReferences
BRAY F, FERLAY J, SOERJOMATARAM I, et al. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin, 2018, 68(6): 394-424. doi: 10. 3322/caac.21492.
CHEN Y P, CHAN A T C, LE Q T, et al. Nasopharyngeal carcinoma. Lancet, 2019, 394(10192): 64-80. doi: 10.1016/S0140-6736(19)30956-0.
LEE A W M, MA B B Y, NG W T, et al. Management of nasopharyngeal carcinoma: current practice and future perspective. J Clin Oncol, 2015, 33(29): 3356-3364. doi: 10.1200/JCO.2015.60.9347.
HAN B, CHAO J, YAO H. Circular RNA and its mechanisms in disease: from the bench to the clinic. Pharmacol Ther, 2018, 187: 31-44. doi: 10. 1016/j.pharmthera.2018.01.010.
WANG L, ZHANG Z, TIAN F. Circ-ITCH regulates nasopharyngeal carcinoma progression via miR-224-3p/ITCH axis. Eur Rev Med Pharmacol Sci, 2020, 24(13): 7287-7295. doi: 10.26355/eurrev_ 202007_21887.
LIU X, LIU F, LI J, et al. CircADARB1 promotes nasopharyngeal carcinoma progression by enhancing ZEB1 expression. Cancer Sci, 2021, 112(5): 1849-1861. doi: 10.1111/cas.14856.
WANG W, LI Y, LI X, et al. Circular RNA FOXP1 promotes hepatocellular carcinoma progression by regulating miR-875-3p/ROCK1 axis. J Cell Mol Med, 2020, 24(12): 6789-6800. doi: 10.1111/jcmm.15328.
XIANG Y, LV D, SONG X, et al. CircFOXP1 promotes gastric cancer progression by sponging miR-942-5p and regulating BCL2 expression. Front Oncol, 2021, 11: 647045. doi: 10.3389/fonc.2021.647045.
SHEN Z, ZHOU L, ZHANG C, et al. CircFOXP1 promotes non-small cell lung cancer progression by regulating miR-185-5p/IGF2 axis. Thorac Cancer, 2021, 12(10): 1533-1542. doi: 10.1111/1759-7714.13930.
SALMENA L, POLISENO L, TAY Y, et al. A ceRNA hypothesis: the Rosetta Stone of a hidden RNA language? Cell, 2011, 146(3): 353-358. doi: 10.1016/j.cell.2011.07.014.
ZHENG H, ZHANG W, ZHANG Y, et al. MiR-4429 suppresses gastric cancer progression by targeting PBX3. J Cancer, 2020, 11(11): 3207-3217. doi: 10.7150/jca.41329.
ZHOU H, LIU X, LIU Y, et al. MiR-4429 inhibits lung adenocarcinoma cell proliferation and colony formation via Wnt/β-catenin pathway. J Thorac Dis, 2021, 13(5): 2987-2996. doi: 10.21037/jtd-21-723.
CHUA M L K, WEE J T S, HUI E P, et al. Nasopharyngeal carcinoma. Lancet, 2016, 387(10022): 1012-1024. doi: 10.1016/S0140-6736(15)00055-0. ZHONG Y, DU Y, YANG X, et al. Circular RNAs function as ceRNAs to regulate and control human cancer progression. Mol Cancer, 2018, 17(1): 79. doi: 10.1186/s12943-018-0827-8.
WANG S, ZHANG Y, CAI Q, et al. Circular RNA FOXP1 promotes gallbladder cancer progression by sponging miR-370-3p and regulating EZH2 expression. Cancer Cell Int, 2020, 20: 456. doi: 10.1186/s12935-020-01543-5.
LUO L, ZHANG J, TANG H, et al. CircFOXP1 promotes nasopharyngeal carcinoma progression via miR-143-3p/ROCK1 axis. Onco Targets Ther, 2021, 14: 1987-2000. doi: 10.2147/OTT.S297832.
THIERY J P, ACLOQUE H, HUANG R Y, et al. Epithelial-mesenchymal transitions in development and disease. Cell, 2009, 139(5): 871-890. doi: 10.1016/j.cell.2009.11.007.
CHEN P, WANG H, DUAN X, et al. MiR-4429 suppresses esophageal squamous cell carcinoma progression by targeting SRXN1. J Cancer, 2021, 12(14): 4221-4231. doi: 10.7150/jca.58234.
PAN X, ZHANG X, GONG J, et al. MiR-4429 inhibits clear cell renal cell carcinoma progression by targeting SRXN1. J Cancer, 2020, 11(19): 5754-5764. doi: 10.7150/jca.45678.
LI J, SUN D, PU W, et al. Circular RNAs in cancer: biogenesis, function, and clinical significance. Cell Death Dis, 2020, 11(6): 426. doi: 10.1038/s41419-020-2634-2.
Refbacks
- There are currently no refbacks.
