Effects of LingQiJuanGan Capsule Medicated Serum on Apoptosis of Activated Rat Hepatic Stellate Cells

QIN Wen, DENG Cun-liang, CHEN Wen. et al

Abstract

To investigate the effects of LingQiJuanGan Capsule medicated serum on the apoptosis of and the expression of Bcl-2/Bax in hepatic stellate cell-T6 (HSC-T6) activated by platelet-derived growth factor (PDGF). Methods Preparation of drug-medicated serum. 30 SD rats were randomly divided into three groups. Serum was obtained from the SD rats administrated intragastricly with saline (10 mL/kg, group A), Fufang Biejia Ruangan Tablet solution (1.5 g/kg, group B) and LingQiJuanGan Capsule solution respectively (4.25 g/kg, group C). Detection of cell apoptosis and the expression of Bcl-2/Bax. HSC-T6 cells were activated by 10 ng/mL PDGF and incubated with drug-medicated serum at 200 mL/L. Cell apoptosis and the expression of Bcl-2/Bax were measured by flow cytometry at 0, 12, 24 and 48 h. Results Significantly higher apoptotic rates of HSC-T6 in group B and group C were detected at 12, 24, 48 h compared with that of group A (P<0.05 ), wheraus no significant difference was found between group B and group C (P>0.05). With increase of exposure time, the apoptotic rates of HSC-T6 in group B and group C increased (P<0.05). The levels of Bcl-2 expression in HSC-T6 in group B and C were lower than that of group A (P<0.05) at 12, 24, 48 h (No significant difference between group B and group C, P>0.05). The expression level of Bcl-2 decreased with increased exposure time (P>0.05) in both group B and group C.However, no such trend was observed in group A (P>0.05). In both group B and group C, the expression level of Bax increased in a time-dependent manner. Conclusion LingQiJuanGan Capsule can induce apoptosis of PDGF activated HSC-T6 and inhibit hepatic fibrosis in a time-dependent manner by influencing the expression of Bcl-2/Bax.

 

Keywords: Hepatic fibrosis, Seropharmacological method, Apoptosis Bcl-2 Bax 

 

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References


Gabele E, Brenner DA, Rippe RA, et al. Liver fibrosis; signals leading to the amplification of the fibrogenic hepatic stellate cell. Front Biosci,2003; 1(8) :69-77.

Tacke F, Weiskirchen R. Liver fibrosis - pathogenesis and novel therapeutic approaches. Internist (Berl),2010; 51 ( 1) ;21- 29.

Yoshida K, Matsuzaki K. Differential regulation of TGF-£/ Smad signaling in hepatic stellate cells between acute and chronic liver injuries. Front Physiol,2012; 17(4) :83-95.

Das SK, Vasudevan DM. Genesis of hepatic fibrosis and its biochemical markers. Scand J Clin Lab Invest.2008;68(4) :260-325

Guo SG. Zhang W. Jiang T, et al. Influence of serum collected from rat perfuse with compound Biejiaruangan drug on hepatic stellate cells. World J Gastroenterol.2004 ; 10( 10): 1487-1492.

Iwama H, Amagaya S. Ogihara Y. Effect of shoszikoto, a Japanese and Chinese traditional herbal medical mixture on the mitogenic activity of lipopoly saccharide. A new pharmacology testing method. J EthnopharmacoU 1987;21( 1) :45-53.

Gao Y. Gao H. Chan E, et al. Antitumor activity and underlying mechanisms of ganopoly.the refined polysaccharides extracted from Ganoderma lucidum in mice. Immunol Invest. 2005;34(2):171-198.

Fowell AJ, Collins JE. Duncombe DR, etal. Silencing tissue inhibitors of metalloproteinases (TIMPs) with short interfering RNA reveals a role for TIMP-1 in hepatic stellate cell proliferation. Biochem Biophys Res Commun, Life Sci.2011? 407(2);277-282.

Fischer R, Schmitt M. Bode JG. et al. Expression of the peripheraltype benzodiazepine receptor and apoptosis induction in hepatic stellate cells. Gastroenterology. 2001; 120(5): 1212- 1226.


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