Regulatory Effect of Two Component Signal Transduction System saeRS on the Survival of Staphylococcus Epidermidis

LOU Qiang, WANG Yao-hui, QU Di.et al

Abstract

Objective To investigate the survival ability of Staphylococcus epidermidis (S.epidermidis) wildtype (WT), saeRSmutant (SAE) and saeRS complementary (SAEC) strains under different concentrations of glucose. Methods We measured the survival ability, biofilm forming ability, medium acidity and antimicrobial susceptibility of S.epidermidisin medium containing different concentrations of glucose. Results Compared with WT, the survival ability, biofilm forming ability and resistance to antibiotics (such as penicillin, oxacillin, gentamicin, ciprofloxacin and amikacin) of saeRSmutant increased significantly in response to glucose. SAE and SAEC showed the strongest survival ability and biofilm forming ability when grown in medium containing 14 mmol/L glucose and 28 mmol/L, respectively. WT showed no significant different survival and biofilm forming abilities when cultured with various concentrations of glucose. The medium acidity of saeRSmutant (pH=8.07) was lower than the WT (pH=7.0) in the presence of 14 mmol/L glucose. Conclusion saeRS may influence the survival ability of S.epidermidis by affecting glucose utilization.

Keywords: Blucose metabolism, Two component signal transduction system saeRS, Biofilm formation, Staphylococcus epidermidis 


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References


Joshi HM. Tabita FR. A global two component signal transduction system that integrates the control of photosynthesis, carbon dioxide assimilation, and nitrogen fixation. Proc Natl Acad Sci U S A.1996;93(25); 14515-14520.

Benson MA, Lilo S, Nygaard T, et al. Rot and SaeRS cooperate to activate expression of the staphylococcal superantigen-like exoproteins. J Bacteriol, 2012; 194 (16); 4355- 4365.

Lou Q. Qi Y, Ma Y, et al. Two-component signal transduction system saeRS positively regulates staphylococcus epidermidis glucose metabolism. Scientific World J, 2014 Jan 23; 2014: 908121. doi: 10.1155/2014/908121. eCollection 2014.

Cerca F, Franca A, Guimaraes R, et al. Modulation of poly-N- acetylglucosamine accumulation within mature Staphylococcus epidermidis biofilms grown in excess glucose. Microbiol Immunol.2011;55( 10):673-682.

Gotz F. Staphylococcus and biofilms. Mol Microbiol, 2002; 43 (6):1367-1378. 2005;45(3):431-436.

Lou Q. Zhu T, Hu J. et al. Role of the SaeRS two-component regulatory system in Staphylococcus epidermidis autolysis and biofilm formation. BMC Microbiol. 2011 Jun 24; 11: 146. doi: 10. 1186/1471-2180-11-146.


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