Effects of 17β-estradiol on the Expression Caveolin-1 and Type Ⅲ Collagen in the Mouse Lung FibroblastStimulated by SiO 2

Abstract

To observe the effects of different concentration of 17β-estradiol (17β-E2) on the expression of Caveolin-1 and type Ⅲ collagen in the mouse lung fibroblast stimulated by SiO2. Methods Fibroblast cells were devided into five groups: blank control group, SiO2 (100 mg/L) group and SiO2 (100 mg/L)+ different concentration of 17β-E2 (10-8，10-7，10-6 mol/L) groups. After treated with different concentration of 17β-E2, for 48 h, the cells were collected, then the expression of Caveolin-1 and of type Ⅲ collagen were examined with immunohistochemistry method. Results Compared with the blank control group, the expression of Caveolin-1 in mouse lung fibroblasts treated by SiO2 significantly decreased (P <0.05), While which in the SiO2+17β-E2 group significantly increased as 17β -E2 dose increased (P < 0.05); There were significantly different in the expression of type Ⅲ collagen among different groups (P <0.05), which in SiO2 group were evidently higher than that in the blank control group, while which in 17β-E2 groups decreased significantly, when compared with SiO2 group (P <0.05); 17β-E2 increased the expression of Caveolin-1 and decreased the expression of type Ⅲ collagen in the dose dependent manner. Correlation analysis showed that 17β-E2 was positive correlated with the expression of Caveolin-1 (r=0.926, P <0.05), and negative correlated with the express of type Ⅲ collagen (r=-0.914, P <0.05), and the expression of Caveolin-1 and the expression of type Ⅲ collagen was negatively correlation (r=-0.887, P <0.05). Conclusion 17β-E2 may inhibit the expression of type Ⅲ collagen by up-regulating the expression of Caveolin-1 in mouse lung fibroblast cell to play a role in the resistance of the lung fibrosis.

Keywords: 17β-estradiol, Lung fibroblast, Caveolin-1, Type Ⅲ collagen