The Effect of p-JAK1/p-STAT3 Signaling Way in Intervening NAFLD by Blueberry Probiotic Serum

ZHU Juan-juan, CHENG Ming-liang, ZHOU Ming-yu. et al

Abstract

To explore the mechanism of interlukin-22 (IL-22)-mediated phosphor-Janus kinase-1(p-JAK1)/phosphor-signal transducer and activator of transcription 3 (p-STAT3) signaling way in the experiment of improving non-alcholic fatty liver disease (NAFLD) by blueberry probiotic serum.Methods The rat serums with low-, medium-, and high-dose of 10% blueberry probiotics, as well as saline were prepared. NAFLD model was built by inducing normal liver cell line L-02 with free fatty acid (FFA).NAFLD model cells were cultured with saline serum (model group), low-, medium-, and high-dose blueberry probiotics serums (low-, medium-, and high-dose serum groups) ,respectively .Normal liver cell group (normal group) was cultured with saline serum . Oil Red O staining was used to detect the lipid deposition in the cells; the intracellular level of triglyceride (TG) was quantitatively determined; the gene and protein expressions of IL-22, p-JAK1, p-STAT3, sterol-regulatory element binding protein-1c (SREBP-1c ) were detected by RT-PCR, Western blot and immunofluorescence methods. Results Twenty-four hours after modeling, a large amount of lipid deposition could be observed in model group. Compared with normal group, model group showed lower gene and protein expression levels of IL-22, p-JAK1 and p-STAT3 (P SREBP-1c and TG levels (P IL-22, p-JAK1, p-STAT3 and lower SREBP-1c compared with the model, low-, and medium-dose serum groups (P low- andmedium-doseserum groups were found (P >0.05). Conclusion The blueberry probiotics could antagonize the NAFLD via p-JAK1/p-STAT3 signaling way.

 

Keywords: Blueberry probiotics serum, Hepatocyte, Steatosis IL-22, p-JAK1 p-STAT3 

 

 

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References


MARIANA VM. HELENA CP. Non-alcoholic fatty liver disease; what the clinician needs to know? World J Gastroenterol.2014 ,20(36): 12956-12980.

JUAN PA, ROBERTO C, RODRIGO Z, et al. Management of nonalcoholic fatty liver disease: an evidence-based clinical practice review. World J Gastroenterol,2014»20(34): 12182- 12201.

KAREEM H, VARUN B, MOHAMMED EER, et al. Nonalcoholic fatty liver disease: a comprehensive review of a growing epidemic. World J Gastroenterol, 2014, 20 ( 34 ) : 12082-12101.

IYER SS, CHENG G. Role of interleukin 10 transcriptional regulation in inflammation and autoimmune disease. Crit Rev Immunol, 2012,32( 1): 23-63.

MASTELIC B, DO ROSARIO AP, VELDHOEN M. et al. IL-22 protects against liver pathology and lethality of an experimental blood-stage malaria infection. Front Immunol, 2012, 3: 85 [ 2016-03-21 ]. http://journal, frontiersin. org/ article/10. 3389/fimmu. 2012. 00085/full. doi; 10. 3389/ fimmu. 2012.00085.

CHESTOVICH PJ, UCHIDA Y, CHANG W, et al. Interleukin-22; implications for liver ischemia-reperfusion injury. Transplantation, 2012,93(5); 485-492.

KISH, PARK O, ZHENG M, et al. Interleukin-22 treatment ameliorates alcoholic liver injury in a murine model of chronic-binge ethanol feeding; role of signal transducer and activator of transcription 3. Hepatology, 2010, 52 (4 ): 1291- 1300.

CHALASANI N, YOUNOSSI Z, LA VINE JE, et al. The diagnosis and management of non-alcoholic fatty liver disease: practice Guideline by the American Association for the Study of Liver Diseases, American College of Gastroenterology, and the American Gastroenterological Association. Hepatology,2012,55(6); 2005-2023.

WANG Y. CHENG M, ZHANG B, et al. Dietary supplementation of blueberry juice enhances hepatic expression of metallothionein and attenuates liver fibrosis in rats. PLoS One, 2013, 8 ( 3 ); e58659 [2016-03-06]. lutp:// dx. doi. org/10. 1371/journal, pone. 0058659.

REN T. HUANG C, CHENG M. Dietary blueberry and bifidobacteria attenuate nonalcoholic fatty liver disease in rats by affecting SIRTl-mediated signaling pathway. Oxid Med Cell Longev,2014, 2014 : 465059 [2016-03-06]. https://dx. doi. org/10. 1155/2014/469059.

OSMAN N, ADAWI D, AHRNE S, et al. Endotoxin- and D-galactosamine-induced liver injury improved by the administration of Lactobacillus. Bifidobacterium and blueberry. Dig Liver Dis,2007,39(9) ;849-856.

WU М, WANG Н. SHI J. et al. Gene expression profiles identify both MyD88-independent and MyD88-dependent pathways involved in the maturation of dendritic cells mediated by heparan sulfate: a novel adjuvant. Hum Vaccin Immunother,2014,10( 12):3711-3721.

YANG L, ZHANG Y, WANG L, et al. Amelioration of high fat diet induced liver lipogenesis and hepatic steatosis by interleukin-22. J Hepatol.2010.53(2) :339-347.

YANG L, ZHANG Y. WANG L, et al. Amelioration of high fat diet induced liver lipogenesis and hepatic steatosis by interleukin-22. J Hepatol.2010.53(2) :339-347.

KONG X. FENG D, MATHEWS S. et al. Hepatoprotective and anti-fibrotic functions of interleukin-22; therapeutic potential for the treatment of alcoholic liver disease. J Gastroenterol Hepatol.2013.28 (Suppl 1):56-60.

ESLAMPARAST T. POUSTCHI H, ZAMANI F, et al. Synbiotic supplementation in nonalcoholic fatty liver disease;a randomized, double-blind, placebo-controlled pilot study. Am J Clin Nutr.2014.99(3):535-542.

FERNANDES I. MARQUES F. FREITAS VD. et al. Antioxidant and anti-proliferative properties of methylated metabolites of anthocyanins. Food Chem.2013.141 (3):2923- 2933.

VENDRAMES, DAUGHERTY A, KRISTO AS, et al. Wild blueberry ( Vaccinium anguslifolium ) consumption improves inflammatory status in the obese Zucker rat model of the metabolic syndrome. J Nutr Biochem. 2013 , 24 ( 8 ): 1508-1512.

BRANNING C. HAKANSSON A, AHRNE S. et al. Blueberry husks and multi-strain probiotics affect clolnic fermentation in rats. Br J Nutr.2009 .101 (6) :859-870.


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