Effect of Recombinant Antigen Ferritin on DC Induce Immune Response

Abstract

To explore the immunoreaction against protoscolex infection by the recombinant ferritin of echinococcus granulosus （rEg.ferritin） immunized mice. MethodsBone marrow deriedstem cells (BMDCs) were collected and exposed in different antigens by cell culture and included 6 groups, there were hydatid fluid （HF） group (HF 30 μg/mL), rEg.ferritin group (rEg.ferritin 1 μg/mL)，rEg.ferritin+LPS group (rEg.ferritin 1 μg/mL and LPS 100 ng/mL), HF+LPS group (HF 30 μg/mL and LPS 100 ng/mL), LPS group (LPS 100 ng/mL) and control (not add antigen). Morphology of BMDCs in different groups were detected by scan electron microscope (SEM). Phagocytic-ability, T cell multiplication capability and surface marker expression of BMDCs in different groups were detected by flow cytometry.Cytokines of Th1 〔interleukin (IL)-12p70, tumor necrosis factor-α (TNF-α)〕 and Th2 (IL-6 and IL-10) in surpernatant of BMDCs in different groups were detected by enzyme-linked immunosorbent assay (ELISA). Results rEg.ferritin or rEg.ferritin+LPS stimulated BMDCs maturation，which the dendrites number of BMDCs in these 2 groups were more and longer than control , HF and HF+LPS groups ( P<0.05)，and induced higher levels of surface molecules major histocompatibility complexⅡ (MHCⅡ), CD80, CD86 and CD40, also had strong ability to induce T cell multiplication than control, HF, HF+LPS groups ( P<0.05), but had weak phagocytosis than HF group, and secreted higher levels of IL-6, IL-12p70, TNF-α and IL-10 than control group. ConclusionBMDCs are stimulated with rEg.ferritin and became maturation. By activating T cell and releasing more cytokines of Th1 and Th2, rEg.ferrtin can induce BMDCs to produce Th1 and Th2 immunoreaction.

Keywords: Recombinant antigen ferritin, Dendritic cellImmunoreaction