Effects of MSP on Cell Cycle and EMT of Non-Small Cell Lung Cancer PC14

SHI Xue-ni, WEI Shi-hang, PENG Xu. et al

Abstract

To study the effect of macrophage stimulating protein (MSP) on the cell cycle of non-small cell lung cancer PC14 cells without expression of recepteur d’originenanta (RON) and MSP, and analyse its effect on PC14’s epithelial mesenchymal transition (EMT) capacity. MethodsVitro culture PC14 (blank control), PC14-Mst1-pEGFP-N1 (stablely expressed MSP) and PC14-pEGFP-N1. Cell cycles were detected by flow cytometry and the gaps between cells during growth were measured by transmission electron microscope (TEM); RT-PCR and Western blot were used to figure out the shifts of EMT related gene expression in PC14-Mst1-pEGFP-N1 cells. ResultsCompared with the PC14 group and PC14-pEGFP-N1 group, PC14-Mst1-pEGFP-N1 population of G1/G0 phase were significantly increased while S and G2/M phase were significantly reduced;The gaps between PC14-Mst1-pEGFP-N1 cells decreased; RT-PCR and Western blot showed that mRNA and protein levels of E-cadherin of PC14-Mst1-pEGFP-N1 were significantly higher than that of PC14, but mRNA and protein levels of Vimentin were significantly lower. ConclusionMSP may affect the cell cycle of PC14 and inhibit its EMT procedure by regulating the expression of related proteins including E-cadherin and Vimentin when RON was not expressed.

 

Keywords: Macrophage stimulating protein, PC14 cell, Epithelial mesenchymal transition, Cell cycle 

 

 

Full Text:

PDF


References


LI J, CHANDA D, SVERDLOV R, et al. MSP: an emerging player in metabolic syndrome. Cytokine Growth Factor Rev,2015,26( 1);75-82.

SCANLON CS, VANTUBERGEN EA, D9 SILVA NJ. Biomarkers of epithelial-mesenchymal transition in squamous cell carcinoma. Dent Res,2013,92(2); 114-121.

WANG MH, WANG D, CHEN YQ. Oncogenic and invasive potentials of human macrophage-stimulating protein receptor, the RON receptor tyrosine kinase. Carcinogenesis, 2003, 24 (8);1291-1300,

WANG MH, LEE W, LUO YL, et al. Altered expression of the RON receptor tyrosine kinase in various epithelial cancers and its contribution to tumourigenic phenotypes in thyroid cancer cells. J Pathol,2007,213(4);402-411.

CAMPER, YANG A, GRAY MJ, et al. Tyrosine kinase receptor RON in human pancreatic cancer. Cancer.2007.109 (6):1130-1139.

СОТЁМ. MILLER AD, LIU SL. Human RON receptor tyrosine kinase induces complete epithelial-to-mesenchymal transition but causes cellular senescence. Biochem Biophy Res Commun,2007,360(0:219-225,

WANG MH. GONIAS SL. SKEEL A. et al. Proteolytic activation of single-chain precursor macrophage-stimulating protein by nerve growth factor-gamma and epidermal growth factor-binding protein, members of the kallikrein family. J Biol Chem, 1994,269(19) : 13806-13810.

IWAMAL A, YAMAGUCHI N, SUDA T. STK/RON receptor tyrosine kinase mediates both apoptotic and growth signals via the multifunctional docking site conserved among the HGF receptor family. ЕМВОЛ996,5(2); 15866-15875.


Refbacks

  • There are currently no refbacks.