Expression of FOXC-2 and YB-1 in Gastric Carcinoma and Its Role in Invasion and Metastasis

JIN Xin, QIAN Jun, YU Da-jun. et al

Abstract

To investigate the expression of FOXC-2, YB-1 and related proteins and their influences on development, invasion and metastases in gastric carcinoma.MethodsA total of 193 tissue samples were collected, including 50 cases of normal gastric mucosa,50 cases of gastric mucosal intraepithelial neoplasia and 93 cases of primary gastric carcinoma. The 93 cases of primary gastric carcinoma included 74 cases of positive lymph node metastasis tissues,19 cases of nonmetastasis tissues and 33 cases of distant metastasis tissues. Immuohistochemistry was used to detect the expression and distribution of FOXC-2, YB-1, E-cadherin,Vimentin and MMP-2 in normal and intraepithelial neoplasia, gastric carcinoma, positive lymph node metastasis tissues and distant metastasis tissues. ResultsThe expressions of FOXC-2, YB-1, Vimentin and MMP-2 in gastric carcinoma were significantly higher than those in normal and intraepithelial neoplasia while the expression of E-cadherin was significantly lower (P<0.05).The expressions of FOXC-2, YB-1 were significantly correlated with low expression of E-cadherin and high expression of Vimentin and MMP-2 (P<0.05). The expression of FOXC-2 protein was significantly correlated with TNM stage, lymph node metastasis and distant metastases (P<0.05).The expression of YB-1 protein was significantly correlated with TNM stage, differentiation degree, invasion depth, lymph node metastasis and distant metastasis (P<0.05). The expression of MMP-2 protein was closely related to the degree of differentiation, invasion depth, lymph node metastasis and distant metastasis (P<0.05). ConclusionFOXC-2,YB-1 may be related to the occurrence,development,invasion and metastasis of gastric carcinoma. The possible mechanism is to promote the invasion and metastasis of cancer cells by activating the epithelial mesenchymal transition process and up regulating the expression of MMP-2.

 

Keywords: Gastric carcinoma, FOXC-2YB-1MMP-2, Epithelial-mesenchymal transition, Metastasis 

 

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