Effect of Rutin on Liver Function and Morphology in Type 1 Diabetes Mice Induced by Streptozotocin
Abstract
To investigate the effect of Rutin on the function and morphology of liver in type 1 diabetes mice induced by streptozotocin (STZ). Methods Type 1 diabetic mice model was established with the treatment of STZ by a 5 d intraperitoneal administration to male Kunming mice. Normal group had 12 mice without treatment of STZ, the mice with DM after STZ treated were randomly divided into DM group, low-dose（50 mg/kg） Rutin group and high-dose （100 mg/kg） Rutin group, each group had 12 mice. The mice in normal and DM group were given sodium carboxymethyl cellulose (10 mg/kg). Each mice was given above drugs by intragastric administration for 8 weeks. Postprandial random blood glucose was measured at 4 weeks and 8 weeks and the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), total protein (TP) and albumin (ALB) in serum were detected by automatic biochemical analyzer after 8 weeks. The morphology of liver was observed by HE and Masson staining. The ultrastructure of liver tissue was observed by electron microscope. Results After a continuous small-dose injection of STZ, the success rate of diabetes model mice were up to 98%. The blood glucose of the model group was significantly increased (P<0.01), and the levels of ALT, AST and LDH in serum were significantly higher, and TP and ALB were lower than those in normal group (P<0.05, P<0.01). Compared to the DM group, the levels of blood glucose were lower (P<0.05) at 4 weeks and 8 weeks, the contents of ALT, AST and LDH were significantly decreased, and TP and ALB were improved in both Rutin dose group. High-dose group performed more obvious (P<0.05, P<0.01). Morphological observation showed the tissue morphology of Rutin treatment group were improved obviously, and the effect was more significant in high-dose Rutin group. Conclusion Rutin may improve the liver function and reduce the damage of liver tissue in STZ-induced type 1 diabetic mice.
Keywords: Rutin, Type 1 diabetes, Liver function, Morphology
Full Text:
PDFReferences
YIN J.ZENG F.WU N,et al. Interleukin-8 promotes human ovarian cancer cell migration by epithelial-mesenchymal transition induction in vitro. Clin Transl Oncol,2015» 17(5) ; 365-370.
ACLOQUE H, THIERY JP. NIETO МA. The physiology and pathology of the EMT. Meeting on the epithelial- mesenchymal transition. EMBO Rep,2008,9(4) ;322-326.
TAMURA S.SHIOZAKI H. MIYATA M, et al. Decreased E-cadherin expression is associated with haematogenous recurrence and poor prognosis in patients with squamous cell carcinoma of the oesophagus. Br J Surg, 1996,83( 11); 1608- 1614.
ZHANG Y, AN J, LV W, et al. miRNA-129-5p suppresses cell proliferation and invasion in lung cancer by targeting microspherule protein 1, E-cadherin and vimentin. Oncol Lett,2016,12(6) :5163-5169.
VILLA GUZMAN J C. ESPINOSA J, CERVERA R, et al. Gastric and colon metastasis from breast cancer; case report, review of the literature, and possible underlying mechanisms. Breast Cancer (Dove Med Press) ,2017,9 ; 1-7.
CHOI SH,KWON OJ .PARK JY,et al. Inhibition of tumour angiogenesis and growth by small hairpin HIF-1 alpha and IL- 8 in hepatocellular carcinoma. Liver Int. 2014 . 34 ( 4 ): 632- 642.
WUS.SHANG H. CUI L. et al. Targeted blockade of interleukin-8 abrogates its promotion of cervical cancer growth and metastasis. Mol Cell Biochem. 2013. 375 ( 1-2): 69-79.
PINE SR, MECHANIC LE, ENEWOI-D L, et al. Increased levels of circulating interleukin 6, interleukin 8, C-reactive protein, and risk of lung cancer. J Natl Cancer Inst, 2011, 103(14);1112-1122.
YIN J, YU C, YANG Z. et al. Tetramethylpyrazineinhibits migration of SKOV3 human ovarian carcinoma cells and decreases theexpression of interleukin-8 via the ERK1/2, p38 and AP-1 signaling pathways. Oncol Rep,2011 ,26:671 -9.
YIN J, ZENO F, WU N, et al. Interleukin-8 promotes human ovarian cancer cell migration by epithelial- mesenchymal transition induction in vitro. Clin Transl Oncol, 2015,17(5):365-370.
GIACCO F, BROWNLEE WM. Oxidative stress and diabetic complications. Circ Res,2010,107(9); 1058-1070.
ZHANG BN, HAN SY, WANG ZL, et al. Protective effects of rutin from buckwheat flowers and leaves on diabetic cardiomyopathy. Lat Am J Phar,2013 ,32(5) ;674-678.
HABTEMARIAM S. LENTINI G. The therapeutic potential of rutin for diabetes; an update. Mini Rev Med Chem»2015» 15(7);524-528.
HOSSEINZADEH H, NASSIRI-ASL M. Review of the protective effects of rutin on the metabolic functon as an important dietary flavonoid. J Endocrinol Invest, 2014, 37 (9):783-788.
HABTEMARIAM S. Rutin as a natural therapy for Alzheimer’s disease: insights into its mechanism of action. Curr Med Chem,2016,23(9) :860-873.
MOHAMED МН. NAIF ОА, ALI RA, et al. Hepato- protective effect of rutin via IL-6/STAT3 pathway in CC14- induced hepatotoxicity in rats. Biol Res,2015 ,48( 1); 30-36.
Refbacks
- There are currently no refbacks.
