Autophagy Promoted by Pakinson’s Disease Related Protein Pink1
Abstract
To investigate the function of Parkinson's disease(PD)-related protein Pink1 in autophagy. Methods Pink1, autophagy inhibitor Bcl-XL and autophagy induced-factor Beclin1 were amplified with RT-PCR and contructed into pcDNA3.1(+)vector. The stable HEK293 cell line of Pink1 expression was established through the lentivirus system. Pink1, Bcl-XL and Beclin1 were tansfected into the HEK293 Cell Line. Co-Immunoprecipitation and Western blot were performed to determine the interaction between Pink1 and Bcl-XL, the effect of Pink1 on the interaction between Bcl-XL and Beclin1,and the function of Pink1 in autophagy. Results There was a new interaction between Pink1 and antophagy inhibitor Bcl-XL. Overexpressed Pink1 promoted the dissociation of autophagy induced-factor Beclin1 from Bcl-XL. Overexpressed Pink1 increased the autophagic protein LC3Ⅱ/LC3Ⅰ. Conclusion Pakinson's disease related protein Pink1 promotes the dissociation of autophagy induced-factor Beclin1 from antophagy inhibitor Bcl-XL and promotes autophagy.
Keywords: Pink1, Bcl-XL, Beclin1, Mitochondria, Autophagy, Parkinson's disease
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