Correlational Study of Thyroid-Stimulating Hormone and Salivary Microecology

DONG Ting, LUO Jun-yuan, HUANG Zheng-wei

Abstract

To investigate the relationship between the composition of salivary microecology and thyroid-stimulating hormone (TSH) levels in healthy adults.   Methods  Healthy subjects were included in the high-TSH group (n=22, 3.00-4.20 mIU/L) and the low-TSH group (n=24, 0.60-1.80 mIU/L) according to their TSH level. Clinical and laboratory examinations were conducted to measure and analyze the relevant clinical and biochemical indicators. Saliva samples were collected in the two groups and microbial genetic profiles were acquired by 16S rDNA sequencing and bioinformatics analysis.  Results  There was no significant difference in the relevant clinical and biochemical indicators between the high-TSH group and the low-TSH group (P>0.05). Individuals with higher TSH levels had higher abundance and species diversity of salivary microbiome. Partial least squares discriminant analysis (PLS-DA) found that the microecology of the the high-TSH group and the low-TSH group (Adonis, P=0.0460) showed obvious differences in β diversity. Wilcoxon rank-sum test and LEFSe analysis showed significant difference in the abundance of Fusobacteriumbetween the high-TSH group and the low-TSH group.  Conclusion  Differences in the composition of microecology were observed in the saliva of healthy subjects with high TSH levels and those with low TSH levels, and the abundance of Fusobacterium showed the most significant difference between the high and low TSH groups.

 

Keywords: Thyroid-stimulating hormone (TSH), Oral microecology, 16S rDNA sequencing

 

Full Text:

PDF


References


UDOVCIC M， PENA R H， PATHAM B， et al. Hypothyroidism and the Heart. Methodist Debakey Cardiovasc J，2017，13(2): 55–59.

RUHLA S， WEICKERT M O， ARAFAT A M， et al. A high normal TSH is associated with the metabolic syndrome. Clin Endocrinol (Oxf)，2010，72(5): 696–701.

GAO L， XU T， HUANG G， et al. Oral microbiomes: More and more importance in oral cavity and whole body. Protein Cell，2018，9(5): 488–500.

GRAVES D T， CORRÊA J D， SILVA T A. The oral microbiota is modified by systemic diseases. J Dent Res，2019，98(2): 148–156.

MATSHA T E， PRINCE Y， DAVIDS S， et al. Oral microbiome signatures in diabetes mellitus and periodontal disease. J Dent Res，2020， 99(6): 658–665.

SI J， LEE C， KO G. Oral microbiota: Microbial biomarkers of metabolic syndrome independent of host genetic factors. Front Cell Infect Microbiol， 2017， 7: 516[2020-10-22]. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736563/. doi: 10.3389/fcimb.2017.00516.

SU X， ZHAO Y， LI Y， et al. Gut dysbiosis is associated with primary hypothyroidism with interaction on gut-thyroid axis. Clin Sci (Lond)， 2020，134(12): 1521–1535.

YAO Z， ZHAO M， GONG Y， et al. Relation of gut microbes and L-thyroxine through altered thyroxine metabolism in subclinical hypothyroidism subjects. Front Cell Infect Microbiol， 2020， 10: 495[2020-11-10]. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7531258/. doi: 10.3389/fcimb.2020.00495.

DONG T， ZHAO F， YUAN K， et al. Association between serum thyroid-stimulating hormone levels and salivary microbiome shifts. Front Cell Infect Microbiol， 2021， 11: 603291[2020-12-17]. https://www.ncbi. nlm.nih.gov/pmc/articles/PMC7952758/. doi: 10.3389/fcimb.2021.603291.

MATTHEWS D R， HOSKER J P， RUDENSKI A S， et al. Homeostasis model assessment: Insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia，1985， 28(7): 412–419.

Integrative HMP (iHMP) Research Network Consortium. The integrative human microbiome project. Nature，2019，569(7758): 641–648.

RAGUSA M， SANTAGATI M， MIRABELLA F， et al. Potential associations among alteration of salivary miRNAs, saliva microbiome structure, and cognitive impairments in autistic children. Int J Mol Sci， 2020，21(17): 6203.

FARRELL J J， ZHANG L， ZHOU H， et al. Variations of oral microbiota are associated with pancreatic diseases including pancreatic cancer. Gut， 2012，61(4): 582–588.

ZHAO F， DONG T， YUAN K Y， et al. Shifts in the bacterial community of supragingival plaque associated with metabolic-associated fatty liver disease. Front Cell Infect Microbiol， 2020， 10: 581888[2020-12-17]. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7770214/. doi: 10.3389/fcimb.2020.581888.

BHUSHAN B， YADAV A P， SINGH S B， et al. Diversity and functional analysis of salivary microflora of Indian Antarctic expeditionaries. J Oral Microbiol， 2019， 11(1): 1581513[2020-12-17]. https://www.ncbi.nlm.nih. gov/pmc/articles/PMC6394331/. doi: 10.1080/20002297.2019.1581513.

FAUST K， RAES J. Microbial interactions: From networks to models. Nat Rev Microbiol，2012，10(8): 538–550.

RATZKE C， BARRERE J， GORE J. Strength of species interactions determines biodiversity and stability in microbial communities. Nat Ecol Evol，2020，4(3): 376–383.

KOLENBRANDER P E， PALMER R J， Jr， PERIASAMY S， et al. Oral multispecies biofilm development and the key role of cell-cell distance. Nat Rev Microbiol，2010，8(7): 471–480.

GHOLIZADEH P， ESLAMI H， YOUSEFI M， et al. Role of oral microbiome on oral cancers, a review. Biomed Pharmacother，2016，84: 552–558.

HONG B Y， SOBUE T， CHOQUETTE L， et al. Chemotherapy-induced oral mucositis is associated with detrimental bacterial dysbiosis. Microbiome，2019，7(1): 66.

KRUSE A B， KUERSCHNER A C， KUNZE M， et al. Association between high risk for preterm birth and changes in gingiva parameters during pregnancy—A prospective cohort study. Clin Oral Investig，2018， 22(3): 1263–1271.

BRENNAN C A， GARRETT W S. Fusobacterium nucleatum—Symbiont, opportunist and oncobacterium. Nat Rev Microbiol，2019，17(3): 156–166.

KOSTIC A D， GEVERS D， PEDAMALLU C S， et al. Genomic analysis identifies association of Fusobacterium with colorectal carcinoma. Genome Res，2012，22(2): 292–298.

JUN J E， JEE J H， BAE J C， et al. Association between changes in thyroid hormones and incident type 2 diabetes: A seven-year longitudinal study. Thyroid，2017，27(1): 29–38.

B U U， MN S， KM S， et al. Effect of insulin resistance in assessing the clinical outcome of clinical and subclinical hypothyroid patients. J Clin Diagn Res， 2015， 9(2): OC01-4[2020-12-17]. https://www.ncbi.nlm.nih. gov/pmc/articles/PMC4378759/. doi: 10.7860/jcdr/2015/9754.5513.

GURUNG M， LI Z， YOU H， et al. Role of gut microbiota in type 2 diabetes pathophysiology. EBioMedicine， 2020， 51: 102590[2020-12-17]. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6948163/. doi: 10.1016/j. ebiom.2019.11.051.


Refbacks

  • There are currently no refbacks.