Microbial Diversity and Community Analysis of Dental Plaques in Orthodontic Patients Wearing Invisible Appliances and Fixed Appliances

XIE Si-hong, JIANG Qian, CHEN Hong, ZHENG Lei-lei, YANG De-qin

Abstract

To explore the microbial diversity and community structure of dental plaques in orthodontic patients with invisible appliances and fixed appliances and to study the differences.   Methods  Ten orthodontic patients wearing invisible appliances (I) and ten wearing fixed appliances (F) were recruited. Dental plaques were collected from both buccal (B) and lingual (L) sides. Based on 16S rDNA, 40 dental plaque samples were analyzed after Illumina sequencing.   Results  The microbial diversity, abundance and evenness of the FB group were significantly higher than those of the IB and IL groups (P<0.05), while the FL group showed substantial individual differences. The community structures were generally similar among the four groups, but significant differences in the relative abundance of some bacteria were found. The IB group showed higher abundances of Actinomycetes and Rosella (P<0.05), which were considered to be involved in dental caries and periodontal diseases. Some key communities showing significant differences were significantly enriched in the FB group, including Coprobacillus, Bifidobacterium, Enterobacterium, Lactobacillus, etc..   Conclusion  Dental plaques in patients wearing invisible appliances and fixed appliances showed significantly different microbial abundance, diversity and composition, which may be involved in orthodontic complications such as dental caries and periodontal diseases. Orthodontic patients need strengthened measures for oral hygiene maintenance, no matter what kind of appliances they wear.

 

Keywords: Invisible orthodontic, Fixed orthodontic, Dental plaque, Microbiota

 

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References


WANG Q， MA J B， WANG B， et al. Alterations of the oral microbiome in patients treated with the Invisalign system or with fixed appliances. Am J Orthod Dentofacial Orthop，2019，156(5): 633–640.

GUO R， LIU H， LI X， et al. Subgingival microbial changes during the first 3 months of fixed appliance treatment in female adult patients. Curr Microbiol，2019，76(2): 213–221.

ZHAO M， LIU M， CHEN W， et al. Salivary microbial changes during the first 6 months of orthodontic treatment. Peer J， 2020，8: e10446[2022-01-19]. https://doi.org/10.1128/10.7717/peerj.10446.

SUN F， AHMED A， WANG L， et al. Comparison of oral microbiota in orthodontic patients and healthy individuals. Microb Pathog，2018，123: 473–477.

CALLAHAN B J， MCMURDIE P J， ROSEN M J， et al. DADA2: High-resolution sample inference from Illumina amplicon data. Nat Methods， 2016，13(7): 581–583.

JONGSMA M A， PELSER F D， VAN DER MEI H C， et al. Biofilm formation on stainless steel and gold wires for bonded retainers in vitro and in vivo and their susceptibility to oral antimicrobials. Clin Oral Investig，2013，17(4): 1209–1218.

ZAURA E. Next-generation sequencing approaches to understanding the oral microbiome. Adv Dent Res，2012，24(2): 81–85.

GROSS E L， LEYS E J， GASPAROVICH S R， et al. Bacterial 16S sequence analysis of severe caries in young permanent teeth. J Clin Microbiol， 2010，48(11): 4121–4128.

XIAO C， RAN S， HUANG Z， et al. Bacterial diversity and community structure of supragingival plaques in adults with dental health or caries revealed by 16S pyrosequencing. Front Microbiol， 2016， 7: 1145[2022-01-19]. https://doi.org/10.1128/10.3389/fmicb.2016.01145.

DIGE I， RAARUP M K， NYENGAARD J R， et al. Actinomyces naeslundii in initial dental biofilm formation. Microbiology (Reading)， 2009，155(Pt 7): 2116–2126.

TAKAHASHI N. Oral microbiome metabolism: From "Who Are They"?to "What Are They Doing"? J Dent Res，2015，94(12): 1628–1637.

DENG L， ZOU L， WU J， et al. Voriconazole inhibits cross-kingdom interactions between Candida albicans and Actinomyces viscosus through the ergosterol pathway. Int J Antimicrob Agents，2019，53(6): 805–813.

XIA T， BAUMGARTNER J C. Occurrence of Actinomyces in infections of endodontic origin. J Endod，2003，29(9): 549–552.

SHIMADA E， KATAOKA H， MIYAZAWA Y， et al. Lipoproteins of Actinomyces viscosus induce inflammatory responses through TLR2 in human gingival epithelial cells and macrophages. Microbes Infect， 2012，14(11): 916–921.

JAGATHRAKSHAKAN S N， SETHUMADHAVA R J， MEHTA D T， et al. 16S rRNA gene-based metagenomic analysis identifies a novel bacterial co-prevalence pattern in dental caries. Eur J Dent，2015，9(1): 127–132.

SORO V， DUTTON L C， SPRAGUE S V， et al. Axenic culture of a candidate division TM7 bacterium from the human oral cavity and biofilm interactions with other oral bacteria. Appl Environ Microbiol， 2014，80(20): 6480–6489.

BRADSHAW D J， MARSH P D， WATSON G K， et al. Role of Fusobacterium nucleatum and coaggregation in anaerobe survival in planktonic and biofilm oral microbial communities during aeration. Infect Immun，1998，66(10): 4729–4732.


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