Sensitivity Comparison Experiment of Four Testing Methods for Helicobacter pylori
Abstract
To measure with standard microbiology methods the sensitivity of 4 commonly used testing methods for Helicobacter pylori (Hp) and to conduct a comparative study of the correlations and differences across the 4 methods. Methods With the Hp standard strain (SS1) as the reference, colony forming units (CFU) as the units of quantitative analysis for detection performance, and gradient dilution of SS1 suspension as the simulation sample, we measured the sensitivity of 4 Hp testing methods, including bacterial culture, rapid urease test, antigen test, and quantitative fluorescent PCR. CFU values at different concentrations corresponding to the 4 commonly used Hp testing methods were documented and the correlations and differences were analyzed accordingly. Results The sensitivity of Hp bacterial culture, rapid urease test, antigen test and quantitative fluorescent PCR was 2.0×10 CFU/mL, 2.0×105 CFU/mL, 2.0×105 CFU/mL, and 2.0×102 CFU/mL, respectively. Conclusion The testing turnover time and sensitivity of different laboratory methods for Hp testing varied significantly. The quantitative fluorescent PCR and bacterial culture both showed relatively high sensitivity, but bacterial culture has complicated operation procedures and is too time-consuming. The rapid urease test and antigen test both were simple and quick to perform, but showed low sensitivity. For clinical and laboratory testing of Hp, appropriate testing method that can identify the corresponding changes of Hp should be selected according to the actual testing purpose.
Keywords: Helicobacter pylori, Testing methods, Sensitivity
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MARSHALL B J， WARREN J R. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet，1984， 1(8390): 1311–1315.
WANG Y K， KUO F C， LIU C J， et al. Diagnosis of Helicobacter pylori infection: Current options and developments. World J Gastroenterol， 2015，21(40): 11221–11235.
THUNG I， ARAMIN H， VAVINSKAYA V， et al. Review article: the global emergence of Helicobacter pylori antibiotic resistance. Aliment Pharmacol Ther，2016，43(4): 514–533.
HE X J， ZENG X P， JIANG C S， et al. Efficacy and safety of antofloxacin-based triple therapy for Helicobacter pylori eradication failure in China. Dig Dis Sci，2022，67(1): 208–215.
SCHMIDT S T， PEDERSEN G K， CHRISTENSEN D. Rational design and in vivo characterization of vaccine adjuvants. ILAR J，2018，59(3): 309–322.
ROUSSEL Y， WILKS M， HARRIS A， et al. Evaluation of DNA extraction methods from mouse stomachs for the quantification of H. pylori by real-time PCR. J Microbiol Methods，2005，62(1): 71–81.
SHAN J， BAI X， HAN L， et al. Association between atherosclerosis and gastric biomarkers concerning Helicobacter pylori infection in a Chinese healthy population. Exp Gerontol，2018，112: 97–102.
KIM B J， KIM H S， JANG H J， et al. Helicobacter pylori eradication in idiopathic thrombocytopenic purpura: A meta-analysis of randomized trials. Gastroenterol Res Pract， 2018， 2018: 6090878[2021-06-21]. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6198559/. doi: 10.1155/2018/ 6090878.
YANG F， XU Y L， ZHU R F. Helicobacter pylori infection and the risk of colorectal carcinoma: A systematic review and meta-analysis. Minerva Med，2019，110(5): 464–470.
WANG B N， ZHANG J， CHEN S H， et al. Helicobacter pylori and gastrointestinal and neurological diseases: Study protocol of an umbrella review of systematic reviews and meta-analyses. Medicine (Baltimore)， 2019， 98(52): e18460[2021-06-21]. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6946528/. doi: 10.1097/MD.0000000000018460.
QARIA M A， QUMAR S， SEPE L P， et al. Cholesterol glucosylation-based survival strategy in Helicobacter pylori. Helicobacter， 2021， 26(2): e12777[2021-06-21]. https://onlinelibrary.wiley.com/doi/10.1111/hel. 12777. doi: 10.1111/hel.12777.
SHI Y， LIU X F， ZHUANG Y， et al. Helicobacter pylori-induced Th17 responses modulate Th1 cell responses, benefit bacterial growth, and contribute to pathology in mice. J Immunol，2010，184(9): 5121–5129.
HAZELL S L， MARKESICH D C， EVANS D J， et al. Influence of media supplements on growth and survival of Campylobacter pylori. Eur J Clin Microbiol Infect Dis，1989，8(7): 597–602.
MIENDJE V Y， VAN B C， FONTAINE V. Comparative evaluation of 3 selective media for primary isolation of Helicobacter pylori from gastric biopsies under routine conditions. Diagn Microbiol Infect Dis，2010， 68(4): 474–476.
PERETZ A， ON A， KOIFMAN A， et al. An efficiency comparison between three invasive methods for the diagnosis of Helicobacter pylori infections: Culture from stomach biopsy, rapid urease test (CUTest®), and histologic examination of gastric biopsy. Ann Clin Lab Sci，2015，45(2): 148–151.
PATEL S K， PRATAP C B， JAIN A K， et al. Diagnosis of Helicobacter pylori: What should be the gold standard? World J Gastroenterol，2014， 20(36): 12847–12859.
LANGNER M， MACHADO R S， PATRICIO F R， et al. Evaluation of gastric histology in children and adolescents with Helicobacter pylori gastritis using the Update Sydney System. Arq Gastroenterol，2009，46(4): 328–332.
Campylobacter-like organisms in the stomach of patients and healthy individuals. Lancet， 1984， 1(8390): 1348–1349.
BRANDI G， BIAVATI B， CALABRESE C， et al. Urease-positive bacteria other than Helicobacter pylori in human gastric juice and mucosa. Am J Gastroenterol，2006，101(8): 1756–1761.
NISHIKAWA K， SUGIYAMA T， KATO M， et al. A prospective evaluation of new rapid urease tests before and after eradication treatment of Helicobacter pylori, in comparison with histology, culture and 13C-urea breath test. Gastrointest Endosc，2000，51(2): 164–168.
SEO J H， PARK J S， RHEE K H， et al. Limitations of urease test in diagnosis of pediatric Helicobacter pylori infection. World J Clin Pediatr， 2015，4(4): 143–147.
GODBOLE G， MEGRAUD F， BESSEDE E. Review: Diagnosis of Helicobacter pylori infection. Helicobacter， 2020， 25 (Suppl 1): e12735[2021-06-21]. https://onlinelibrary.wiley.com/doi/10.1111/hel. 12735. doi: 10.1111/hel.12735.
VAIRA D， MALFERTHEINER P， MEGRAUD F， et al. Diagnosis of Helicobacter pylori infection with a new non-invasive antigen-based assay. HpSA European study group. Lancet，1999，354(9172): 30–33. KAKIUCHI T， OKUDA M， HASHIGUCHI K， et al. Evaluation of a novel stool antigen rapid test kit for detection of Helicobacter pylori infection. J Clin Microbiol， 2019， 57(3): e01825-18[2021-06-21]. https://doi.org/10.1128/JCM.01825-18.
KAZEMI E， KAHRIZI D， MORADI M T， et al. Association between Helicobacter pylori hopQI genotypes and human gastric cancer risk. Cell Mol Biol (Noisy-le-grand)，2016，62(1): 6–9.
HSIEH M S， LIU C J， HSU W H， et al. Gastric juice-based PCR assay: An alternative testing method to aid in the management of previously treated Helicobacter pylori infection. Helicobacter， 2019， 24(2): e12568[2021-06-21]. https://onlinelibrary.wiley.com/doi/full/10.1111/hel. 12568. doi: 10.1111/hel.12568.
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