Expressions of Spinal Macrophage Colony Stimulating Factor and Its Receptor CSF-1R in the Development ofComplicated Regional Pain Symptom Ⅰ

LIAO Zhi-min, TANG Yu-ying, ZHENG Yang-chun. et alY。

Abstract

To study the changes of mechanical allodynia and temperature hyperalgesia, as well as the expression of the spinal macrophage colony stimulating factor (M-CSF) and its receptor CSF-1R during the development of complicated regional pain symptomⅠ(CRPSⅠ). Methods The animal model of CRPSⅠ was established using prolonged ischemia-reperfusion injury of rodent left hindpaw. The mechanical allodynia and temperature hyperalgesia of ipsilateral hindpaw were continuously measured for 14 d after reperfusion, and the expressions of spinal M-CSF and CSF-1R in ipsilateral spinal cord horn were measured with immunofluorescence technique on day 3, day 7 and day 14 after reperfusion. Results The thresholds of mechanical allodynia and temperature hyperalgesia of ipsilateral hindpaw were significantly decreased (P<0.05). M-CSF was secreted by the astrocytes. CSF-1R was primarily distributed on the microglia. The immunofluorescence intensities of M-CSF and CSF-1R in ipsilateral spinal cord horn were significantly increased on day 7 and day 14 after reperfusion (P<0.05). Conclusion The ischemia-reperfusion injury simulated pain syndrome in CRPSⅠand increased the expressions of spinal M-CSF and CSF-1R.

 

Keywords: Complicated regional pain symptom Ⅰ, Macrophage colony stimulating factor, Microglia 

 

Full Text:

PDF


References


BRUEHL S. An update on the pathophysiology of complex regional pain syndrome. Anesthesiology. 2010, 113(3): 713- 725.

CALVO M. BENNETT DL. The mechanisms of microgliosis and pain following peripheral nerve injury. Exp Neurol.2012,234(2) :271-282.

JI RR. BERTA T, NEDERGAARD M. Glia and pain: is chronic pain a gliopathy? Pain, 2013, 154(Suppl 1):S10-S28 [2015-08-05]. http;//www. ncbi. nlm. nih. gov/pmc/articles/ РМС3858488/. doi; 10. 1016/j. pain. 2013. 06. 022.

SMITH AM. GIBBONS HM. OLDFIELD RL.et al. M-CSF increases proliferation and phagocytosis while modulating receptor and transcription factor expression in adult human microglia. J Neuroinflammation. 2013 ( 10 ): 85. doi: 10. 1186/1742-2094-10-85.

MITRASINOVIC OM. PEREZ GV, ZHAO F. et al. Overexpression of macrophage colony-stimulating factor receptor on microglial cells induces an inflammatory response. J Biol Chem.2001.276(32) :30142-30149.

PYONTECK SM, AKKARI L. SCHUHMACHER AJ, et al. CSF-1R inhibition alters macrophage polarization and blocks glioma progression. Nat Med, 2013, 19 ( 10); 1264- 1272.

VIDYADARAN S, OOI YY, SUBRAMAIAM H, et al. Effects of macrophage colony-stimulating factor on microglial responses to lipopolysaccharide and beta amyloid. Cell Immunol. 2009 ,259(1) s 105-110.

LIU W, XU GZ, JIANG CH, et al. Macrophage colony- stimulating factor and its receptor signaling augment glycated albumin-induced retinal microglial inflammation in vitro. BMC Cell Biol,2011(12) :5. doi: 10.1186/1471-2121-12-5.

CODERRE TJ, XANTHOS DN, FRANCIS ,et al. Chronic post-ischemia pain (CPIP): a novel animal model of complex regional pain syndrome-type I ( CRPS- I ; reflex sympathetic dystrophy ) produced by prolonged hindpaw ischemia and reperfusion in the rat. Pain,2004 ,112( 1/2):94- 105.

KIM YO, KIM IJ, YOON MH. Antiallodynic effect through spinal endothelin-B receptor antagonism in rat models of complex regional pain syndrome. Neurosci Lett, 2015( 584) ; 45-49[2015-08-06]. http://www. sciencedirect. com/science/article/pii/S0304 394014008088. doi:10.1016/j.neulet.2014.10.005.

LIU W, TANG Y. FENG J. Cross talk between activation of microglia and astrocytes in pathological conditions in the central nervous system. Life Sci,2011,89(5/6) : 141-146.

WATKINS LR. HUTCHINSON MR. LEDEBOER A, et al. Glia as the "bad guys”; implications for improving clinical pain control and the clinical utility of opioids. Brain Behav Immun.2007,21(2): 131-146.

ELMORE MR, NAJAFI AR, KOIKE MA, et al. Colony- stimulating factor 1 receptor signaling is necessary for microglia viability, unmasking a microglia progenitor cell in the adult brain. Neuron,2014 ,82(2) ;380-397.

MIKA J, ZYCHOWSKA M, POPIOLEK-BARCZYK К , et al. Importance of glial activation in neuropathic pain. Eur J Pharmacol,2013,716( 1 /2/3); 106-119.


Refbacks

  • There are currently no refbacks.