Trans-differentiation of Human Amniotic Epithelial Cells into Follicle-like Structure

WANG Hui, WANG Fei, BAI Li-ping. et al

Abstract

To determine whether hAECs can differentiate into follicle-like structure. Methods hAECs were isolated from human term placenta and cultured sequentially in medium supplemented with 5% human follicular fluid. The morphology of the cells was examined by inverted microscope. Changes of estradiol levels and expressions of germ cell specific gene in the cultured medium were detected by chemiluminescence immunoassay and Real time-Polymerase Chain Reaction (RT-PCR), respectively. Results Oocyte-like cells (OLCs) were found in those cultured in the medium supplemented with 5% human follicular fluid. The OLCs gradually aggrandized, shrunk and finally disappeared over time. However, this phenomenon was not observed in the control group. Higher expressions ofDAZLandGDF9were found in the follicle group compared with the control which medium without 5% human follicular fluid (P< 0.05), with two peaks. No significant difference in the expression ofSCP3was found between the two groups (P>0.05). Estradiol was detected in the culture medium of the follicle group in a mode of decreasing-rising-decreasing, but not in the control group. Conclusion hAECs have the potential to trans-differentiate into the follicle-like structure in vitro.

 

Keywords: hAECs, Human follicle fluid, Trans-differentiation, Female germ cell, Follicle-like structure Estradiol

 

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References


WHO. Initiative for vaccines research team of the department of immunization, vaccines and biologicals ( 2009 ) Geneva, Switzerland: World Health Organization. 2009. PARKHURST JO. VULIMIRI M. Cervical cancer and the global health agenda: Insights from multiple policy-analysis frameworks. Glob Public Health.2013,8( 10): 1093-1108.

MIKI T, LEHMANN T, CAI H, et al. Stem cell characteristics of amniotic epithelial cells. Stem Cells, 2005, 23(10):1549-1559.

ILANCHERANS, MICHALSKA A, PEH G, et al. Stem cells derived from human fetal membranes display multilineage differentiation potential. Biol Reprod, 2007, 77 (3):577-588.

TEE JY, VAGHJIANI V, LIU YH, et al. Immunogenicity and immunomodulatory properties of hepatocyte-like cells derived from human amniotic epithelial cells. Curr Stem Cell Res Ther,2013,8(1);91-99.

Szukiewicz D, Pyzlak M, Stangret A, et al. Decrease in expression of histamine H2 receptors by human amniotic epithelial cells during differentiation into pancreatic beta-like cells. Inflamm Res,2010,59(Suppl 2) :S205-S207.

JIAWEN S. JIANJUN Z. JIEWEN D, et al. Osteogenic differentiation of human amniotic epithelial cells and its application in alveolar defect restoration. Stem Cells Transl Med,2014,3( 12); 1504-1513.

Niknejad H, Peirovi H, Ahmadiani A, et al. Differentiation factors that influence neuronal markers expression in -vitro from human amniotic epithelial cells. Eur Cell Mater, 2010,

HUBNER К. FUHRMANN G, CHRISTENSON LK, et al. Derivation of oocytes from mouse embryonic stem cells. Science,2003,300(5623): 1251-1256.

DYCEPW, WEN L, LI J. In vitro germline potential of stem cells derived from fetal porcine skin. Nat Cell Biol. Nature cell biology,2006,8(4):384-390.

WANG F. WANG L, YAO X, et al. Human amniotic epithelial cells can differentiate into granulosa cells and restore folliculogenesis in a mouse model of chemotherapy- induced premature ovarian failure. Stem Cell Res Ther,2013. 4(5); 124[2016-11-02]. https;//stemcellres. biomedcentral. com/articles/10. 1186/scrt335. doi; 10. 1186/scrt335.

KEEK, ANGELES VT, FLORES M, et. al. Human DAZL, DAZ and BOULE genes modulate primordial germ¬cell and haploid gamete formation. Nature,2009,462(7270); 222-225.

AZARI M. KAFI M, EBRAHIMI B, et al. Oocyte maturation, embryo development and gene expression following two different methods of bovine cumulus-oocyte complexes vitrification. Vet Res Commun, 2017, 41 ( 1 ): 49- 56.

LI Y« HE Z, SHI S, et al. Scp3 expression in relation to the ovarian differentiation in the protogynous hermaphroditic ricefield eel monopterus albus. Fish physiol Biochem, 2016, 42(6):1609-1619.

GONZALEZ-ORTEGA C, PIN A-AGUILAR RE, CANCINO-VILLAREAL P, et al. Birth after human chorionic gonadotropin-primed oocyte in vitro maturation and fertilization with testicular sperm in a normo-ovulatory patient. J Hum Reprod Sci,2016 ,9(3);210-212.

EGUIZABAL С, MONTSERRAT N. VASSENA R. et al Complete meiosis from human induced pluripotent stem cells Stem cells,2011,29(8): 1186-1195.


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