Effects of Fukang Oral Liquid on the Prevention of Intrauterine Adhesion and Expressions of TGF-β1,PAI-1 and MMP-9 in Endometrium of Rats

HU Sha, LI Ya, MENG Wei-jie, TAN Shi-qiao

Abstract

To investigate the preventing effect of Fukang oral liquid(fuk) in intrauterine adhesions and its effects on the expression of TGFβ-1、PAI-1 and MMP-9 in endometrium of rats with intrauterine adhesions. Methods 50 female wistar rats were divided into high, medium, low dose of Fukang oral liquid group (Hfuk, Mfuk, Lfuk), blank control group (Bcon), and model control group (Mcon) (n=10 in each group). The rats in Hfuk,Mfuk and Lfuk groups were treated with intragastric administration of 4 mL,2 mL and 1 mL Fukang Oral Liquid per day, while the rats in Mcon group and Bcon group received 2 mL physiological saline intragastric administration per day. All of rats were executed on 10th day and the sample of endometrium was harvested for the study of histology and morphology and the expression of TGFβ-1,PAI-1 and MMP-9. Results Under the light microscope, the organizational structure of the uterine cavity and uterine wall was clear in Bcon group, the uterine cavity disappeared in Mcon group, and the layers structure remained normal arrangement in three fuk treated groups. TGFβ-1 and PAI-1 protein expressions in Hfuk,Mfuk,Lfuk groups were less than those in Mcon group (P<0.001), but MMP-9 protein expressions were higher.(P<0.001). Conclusion Fukang oral liquid show preventing effect on IUA,the mechanism may be related to its effects on the expressions of TGF-β1, PAI-1, and MMP-9 in the endometrium.

 

Keywords: Intrauterine adhesions, Fukang oral liquid, Precautions, TGF-β1, PAI-1, MMP-9


Full Text:

PDF


References


Jemal A, Bray F. Center MM, etui. Global cancer statistics. С A Cancer J Clin, 2011 ;61 (2): 69-90.

Hossain MA, Kim DH, Jang JY, et al. Aspirin induces apoptosis in vitro and inhibits tumor growth of human hepatocellular carcinoma cells in a nude mouse xenograft model. InJ Oncol,2012;40(4): 1298-1304.

Morita Y. Hata К, Nakanishi М, et al. Cyclooxygenase-2 promotes tumor lymphangiogenesis and lymph node metastasis in oral squamous cell carcinoma. Int J Oncol,2012;41 (3):885- 892.

Al-Wadei HA, Al-Wadei MH, Ullah MF, et al. Celecoxib and GABA cooperatively prevent the progression of pancreatic cancer in vitro and in xenograft models of stress-free and stress-exposed mice. PLoS One,2012;7(8) :e43376.

Liu H, Yang Y, Xiao J, et al. СОХ-2-mediated regulation of VEGF-C in association with lymphangiogenesis and lymph node metastasis in lung cancer. Anat Rec ( Hoboken), 2010; 293 (11): 1838-1846.

Almhanna K. El-Rayes B. Sethi S.et al. Association between COX-2 expression and effectiveness of COX-2 inhibitors in a phase II trial in patients with metastatic colorectal adenocarcinoma. Anticancer Res«2012;32(8):3559-3563.

Kalim KW, Groettrup M. Prostaglandin Ег inhibits 1L-23 and II.-12 production by human monocytes through down- regulation of their common p40 subunit. Mol Immunol, 2013; 53(3)=274-282.

Patel S. Vetale S. Teli P. et al. 11,-10 production in non-small cell lung carcinoma patients is regulated by ERK. P38 and COX-2. J Cell Mol Med.2012; 16(3) :531-144.

Stolina M. Sharma S. Lin Y. et at. Specific inhibition of cyclooxygenase 2 restores antitumor reactivity by altering the balance of IL-10 and IL-12 synthesis. J Immunol» 2000; 164 (1>:361-370.

Maruyama T, Kono K. Mizukami Y. et al. Distribution of Thl7 cells and FoxP3 ( + ) regulatory T cells in tumor- infiltrating lymphocytes, tumor-draining lymph nodes and peripheral blood lymphocytes in patients with gastric cancer. Cancer Sci. 2010; 101 (9): 1947-1954.

Correale P. Rotundo MS. Del Vecchio MT, et al. Regulatory (FoxP3+) T-cell tumor infiltration is a favorable prognostic factor in advanced colon cancer patients undergoing chemo or chemoimmunotherapy. J Immunother.2010; 33(4):435-441.

Yan M. Jene N. Byrne G, et al. Recruitment of regulatory T cells is correlated with hypoxia-induced CXCR4 expression, and is associated with poor prognosis in basal-like breast cancers. Breast Cancer Res.2011; 13(2):R47.

Morita R. Hirohashi Y, Sato N. Depletion of Tregs in vivo : a promising approach to enhance antitumor immunity without autoimmunity. Immunotherapy.2012;4(11); 1103-1105.

Ramos RN, Oliveira CE, Gasparoto TН, etal. CD25, T cell depletion impairs murine squamous cell carcinoma development via modulation of antitumor immune responses. Carcinogenesis.2012; 33(4);902-929.

Baratelli F, Lin Y, Zhu L. et al. Prostaglandin K2 induces foxp3 gene expression and T regulatory cell function in human CD4- T cell. J Immunol.2005; 175(3) = 1483-1490.

Dlugosova К. Mitasikova М, Bernatova I, et al. Reduced connexin-43 expression in the aorta of prehypertensive rats. Physiol Res.2008;57(SuppI 2):S23-S29.

Li WE. Nagy JI. Connexin43 phosphorylation state and intercellular communication in cultured astrocytes following hypoxia and protein phosphatase inhibition. Kur J Neurosci» 2000;12(7):2644-2650.

Sun CK, Chang I-T. Sheu JJ. et al. Losartan preserves integrity of cardiac gap junctions and PGC-1 alpha gene expression and prevents cellular apoptosis in remote area of left ventricular myocardium following acute myocardial infarction. Int Heart J,2007;48(4):533-546.

Aoki A. Ogawa T. Sumino H. et al. I-ong-term effects of telmisartan on blood pressure . the renin-angiotensin- aldosterone system, and lipids in hypertensive patients. Heart Vessels. 2010; 25( 3): 195-202.

Ichikawa S. Takayama Y. Long-term effects of olmesartan, an Ang II receptor antagonist, on blood pressure and the renin- angiotensin-aldosterone system in hypertensive patients. Hypertens Res.2001;24(6): 641-646.


Refbacks

  • There are currently no refbacks.